Ki-67 antibody (AA 2293-2478)

Catalog No. ABIN7477505

The Mouse Monoclonal anti-Ki-67 antibody has been validated for IHC. It is suitable to detect Ki-67 in samples from Human.

Quick Overview for Ki-67 antibody (AA 2293-2478) (ABIN7477505)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-267M

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 2293-2478

Immunogen: Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil (moderate to strong nuclear staining in 80-90% of the germinal centre B-cells and in the vast majority of the suprabasal squamous epithelial cells).

Negative Control: Liver (nuclear staining reaction only in scattered hepatocytes (<1%), cytoplasmic staining is absent or only weak)

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen,Ki-67 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process